Protective role of vitamin E on the harmful effects of toluene on brain tissue

To determine the protective effects of vitamin E against the damage inflicted by reactive oxygen species during toluene induced brain injury in rats using histopathological, biochemical, and behavioral parameters. Twenty-eight Wistar albino male rats were studied at Hacettepe University Faculty of Medicine Laboratories and Gazi University Faculty of Medicine Metabolism Laboratory in 2006. The rats were divided into 4 groups as follows: control group, toluene only treated group, both toluene and vitamin E treated group, and vitamin E only treated group. Histopathological changes were observed by hematoxylin and eosin staining and the TUNEL method. Serum lipid peroxide levels were measured by the thiobutyric acid method, and the open field test was used for behavioral testing. Our results show that edema between cells in the neuropil, particularly beneath the pia mater, and around congested blood vessels was observed in all groups with different severity. However the pyknotic neurons, in addition to infiltrative cells, were observed in the toluene group to be decreased by the administration of vitamin E, which can be confirmed by the euchromatic nucleated neurons in the cortex of both the toluene and vitamin E treated groups. The levels of malondialdehyde in each group also confirmed these histopathological findings. Significant differences were also found in the open field test between the groups. The differences between the toluene and vitamin E groups in biochemical, histologic, and behavioral examinations, supports the antioxidant protective role of vitamin E against the harmful effect of toluene on the brain

Immunohistochemical evaluation of periprosthetic membrane from loose cemented and uncemented total hip arthroplasty

Evaluate the cellular mechanisms responsible for the aseptic loosening of total hip replacements. Twenty periprosthetic membranes were collected during revision procedures at the Department of Orthopedics, Hacettepe University, Ankara, Turkey, during 1998 to 1999. Arthroplasties with polyethylene components and without polyethylene components were examined under light microscopy and immunohistochemically using the indirect immunoperoxidase technique into 2 different groups. Immunohistochemical analysis with monoclonal antibodies reactive to certain cell surface antigens [CD45, CD44, CD98, CD31, CD26, CD71] revealed different morphological characteristics regarding the adjacent fibrosis, leukocyte activation, and immunologic response. These findings suggested the role of different cellular mechanisms in each group

Fibroblastic reticular cells and fibroblast-like cells determined by monoclonal antibodies B-F45 and B-D46 in humans

Identification of stromal microenvironmental components of lymphoid organs is relatively harder at light microscopic level as few markers, which are mostly not very specific, are available to be used for such a purpose. We screened a large panel to determine monoclonal antibodies [mAbs] those reactive with fibroblasts/fibroblast-like cells aiming to obtain further evidence for the organization and function of this cell group. Tissue samples of forty patients undergoing surgery in Otorhinolaryngology, Obstetrics and Gynecology, Orthopedics and Traumatology, Cardiovascular Surgery and General Surgery Departments, Hacettepe University Medical Faculty Hospital, Ankara, Turkey, due to different pathologies obtained as partial specimens of surgery which were apart from pathological examination were immunostained by indirect immunoperoxidase method in Histology and Embryology department in 2003. Among the screened monoclonal antibodies, monoclonal antibodies B-F45 and B-D46 reacted with the members of the family, therefore examined in detail in available human organs. Among the unique staining patterns of these mAbs, reactivity on fibroblastic reticular cells, perineural sheet cells pericryptal/perivillous fibroblasts were striking. Both mAbs will provide useful tools for further studies on stromal network of peripheral lymphoid organs and peripheral nerves